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United States Department of Agriculture

Agricultural Research Service

Title: Role of Acute Phase Cytokines and Leukocytic Infiltration in a Murine Model of Spirochete-Induced Colitis (Poster Presentation at the Keystone Symposia, Santa Fe, Nm, January 1999)

item Wannemuehler, Michael - IA STATE UNIV., AMES, IA
item Xiaosong, Li - IA STATE UNIV., AMES, IA
item Waters, W. - IA STATE UNIV., AMES, IA
item Sacco, Randy

Submitted to: Keystone Symposia on Molecular and Cellular Biology
Publication Type: Proceedings
Publication Acceptance Date: January 16, 1999
Publication Date: N/A

Technical Abstract: In both pigs and mice, Serpulina hyodysenteriae induces a mucohemorrhagic colitis. The lesions are characterized by mixed inflammatory cell infiltration, edema, gland elongation, and epithelial cell erosion. In addition to the spirochete, the disease requires the presence of a normal microflora and a host inflammatory response. Presently, it was noted that induction of acute phase cytokines (e.g., TNF-alpha, IL-1 beta, MIP-2) are essential fro the induction of lesions. Using semiquantitative RT-PCR, increases in cytokine-specific mRNA, TNF-alpha and MIP-2, were noted within 24 hrs postinfection (PI). Increases in IL-1 beta-specific mRNA was not detected until 4 days PI. Pharmacologic treatment of mice to inhibit the production of the acute phase cytokines, completely blocked the development of colitis without affecting colonization by the spirochete. Cecitis was also abrogated by treatment of mice with monoclonal antibodies [anti-CD18 (R15.7), anti-CD29 (P1D2), or anti-granulocyte (RB6, 8C5)] to block extravasation of or facilitate depletion of inflammatory cells. These results demonstrate the usefulness of this model to define immune mechanisms contributing to mucosal inflammation. (USDA-NRI 9602438)

Last Modified: 4/20/2015
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