|Touchette, K - UNIVERSITY OF MISSOURI|
|Allee, G - UNIVERSITY OF MISSOURI|
|Pace, L - UNIVERSITY OF MISSOURI|
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 1999
Publication Date: N/A
Technical Abstract: Twenty pigs (14 d, 5.6 kg) were used to determine if spray-dried plasma (SDP) affected the pigs' response to an oral E. coli challenge. Weaned pigs were allotted to one of four treatments with two levels of SDP (0 vs 7%) and one of two oral doses (Eschericia coli F17 vs saline). Diets were formulated to contain equal ME and digestible essential amino acids and were fed for 7 d postweaning. On d 6, the pigs were non-surgically fitted with jugular cannulae. On d 7, an oral dose of either saline or LPS was given, followed by blood samples for 10 hr, after which all pigs were killed and intestinal samples were collected to measure villus height, crypt depth, and villus:crypt ratio (VCR). Spray-dried plasma did not affect weight gain. For pigs that received the saline, SDP increased villus height (P<.05), did not affect crypt depth, and increased the villus:crypt ratio (P<.05). Administering an oral dose of E. coli did not affect the intestinal morphology of pigs fed either of the diets. There was an interaction between SDP and E. coli for both serum ACTH (P<.05) and cortisol (P<.001). Serum ACTH for pigs fed SDP was increased by E. coli from 1 to 4 hr after administration, while pigs fed no SDP had no significant increase in serum ACTH. Serum cortisol for pigs fed SDP was increased by E. coli from 1 to 5 hr after administration, while pigs fed no SDP had an increased serum cortisol level from 2.5 to 6 hr after E. coli administration. There were no significant histologic lesions seen in sections of brain, heart, lung, liver, kidney, spleen, or intestinal sections. Our results indicate that feeding SDP alters the responsiveness of the hypothalamic-pituitary-adrenal axis when stimulated by an E. coli challenge.