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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Pathology-Based Techniques for Detection of Viscerotropic Velogenic Newcastle Disease in Chickens

item King, Daniel
item Seal, Bruce

Submitted to: Journal of Comparative Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 8, 1998
Publication Date: N/A

Interpretive Summary: Newcastle disease virus (NDV) is an economically important pathogen of poultry. Outbreaks of virulent forms of the disease result in international trade restrictions. Two different detection methods were tested in an attempt to improve diagnostic identification of the virus in chicken tissues. Using nucleic acid based detection NDV was identified earlier during infection than traditional antibody methods. Consequently, molecular techniques used to identify viruses may be a more sensitive method to use diagnostically.

Technical Abstract: Two pathology based techniques, immunohistochemistry and riboprobe in-situ hybridization, were applied to formalin-fixed, paraffin wax-embedded tissues from chickens infected with three different isolates of velogenic viscerotropic Newcastle disease virus (VVNDV). With the immunohistochemical method, viral protein was consistently detectable in the spleen and cecum at the terminal phase of infection. With in-situ hybridization, viral nucleic acid was consistently detected in the eyelid, spleen and cecum in both the acute and terminal phases. Hybridization with anti-sense probe to detect viral mRNA was often more intense than hybridization to message sense probe to detect viral genomic RNA.

Last Modified: 4/22/2015
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