|Post, Karen - ROLLINS AN.DIS.LAB.,N.C.|
Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 11, 1999
Publication Date: N/A
Technical Abstract: Haemophilus parasuis is a re-emerging pathogen of swine reared in high-health status herds. H. parasuis is the causative agent of Glasser's disease, and is manifested as pleuritis, polyserositis and meningitis. H. parasuis is a commensal of the normal flora of the upper respiratory tract, but under stress and/or infection with other bacterial or viral pathogens, H. parasuis can quickly disseminate into the lower respiratory tract and other tissues. Relatively little is known about the proteins of H. parasuis involved in the immune response to this pathogen. The objective of this study was to identify and characterize immunoreactive proteins of H. parasuis other than the outer membrane proteins for the development of improved vaccines and the development of a "Quick" on-site diagnostic test. By using immunoblotting techniques, a major immunoreactive 46 kDa protein was identified with pooled sera from pigs identified with an active H. parasuis infection. The protein was identified by N-terminal sequencing o internal protein fragments obtained by Lys-C digestion, followed by C-18 reversed-phase HPLC. The identity of the protein was obtained from the H. Influenzae data base. We suggest this protein may serve as a diagnostic antigen for H. parasuis.