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ARS Home » Pacific West Area » Albany, California » Plant Gene Expression Center » Research » Publications at this Location » Publication #108004
Title: SINGLE-COPY TRANSGENIC WHEAT GENERATED THROUGH THE RESOLUTION OF COMPLEX INTEGRATION PATTERNS

Author
item Ow, David
item SRIVASTAVA, V. - UCB-PGEC
item Anderson, Olin

Submitted to: Proceedings of the National Academy of Sciences (PNAS)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/28/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. We demonstrated with wheat transformation that four of four multiple-copy loci could be resolved successfully into single-copy transgenes.

Technical Abstract: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.