ARS | Publication request: DETERMINATION OF TOTAL FAT IN MILK-BASED INFANT FORMULA POWDER BY SUPERCRITICAL FLUID EXTRACTION

Submitted to: Journal of Association of Official Analytical Chemists International
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 1, 2000
Publication Date: January 1, 2003
Citation: Lacroix, D.E., Wolf, W.R. 2003. Determination of total fat in milk-based infant formula powder by supercritical fluid extraction. Journal of Association of Official Analytical Chemists International. 86: 86-95.

Interpretive Summary: The Food Composition Laboratory, U.S. Department of Agriculture, and two commercial laboratories participated in a collaborative study to evaluate the use of a commercially available Supercritical Fluid Extraction(SFE) instrument for doing compositional fat analysis in infant formula. SRM 1846 Infant Formula, commercial milk-based and soy-based infant formula powders were used as representative test samples. Statistical analysis of fat compositional data obtained from all three laboratories showed that Per-Cent fat values obtained are in excellent agreement with the declared label values. Method variability was within the Horwitz "Limits of Acceptability" (+/- 2.8%) for all samples at the level of the analyte analyzed. This collaborative study satisfies all of the requirements for submission to AOAC-International as an AOAC Peer-Verified Method

Technical Abstract: Using a commercially available Supercritical Fluid Extraction(SFE) instrument, a collaborative study involving Food Composition Laboratory, U.S. Department of Agriculture, and two commercial laboratories for the determination of total fat in SRM 1846 Infant Formula, commercial milk-based and soy-based infant formula powders was done. Reproducibility of fat compositional data obtained from all three laboratories showed that Per-Cent fat values obtained are in excellent agreement with the declared label values. The per-cent relative standard deviation were within the Horwitz "Limits of Acceptability" (+/- 2.8%) at the level of the analyte analyzed. Linear Regression Analysis of all matrices spiked with added fat show that the SFE instrument response is due to the added fat only. This collaborative study satisfies all of the requirements for submission to AOAC-International as an AOAC Peer- Verified Method