|Bartleson, Cheryl - IA STATE UNIV., AMES, IA|
Submitted to: Biochemistry and Molecular Biology Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 8, 2000
Publication Date: N/A
Technical Abstract: Haemophilus parasuis is the causative agent of Glaesser's disease, a re- emerging swine respiratory disease in high-health status herds. High- health status herds have resulted from new management practices, such as segregated early weaning. Little is known about the biochemical and physiological characteristics of Haemophilus parasuis. Restriction of iron ncan induce Gram-negative organisms to express new outer membrane proteins, which may function as receptors for an iron-uptake mechanism. Mechanisms used by pathogens to acquire iron in vivo become essential components of the virulence of pathogenesis of the organism. Iron-regulated proteins appear to be conserved within bacterial species; thus, antibodies directed against these proteins could be valuable in protecting the animal from disease. The outer membrane protein profile of Haemophilus parasuis serovar was studied under iron-restricted and iron-repleted conditions. All experiments utilized the iron chelator 2,2'-dipyridyl. Potential iron-regulated outer membrane proteins (IROMPs) were identified by SDS-PAGE analysis. Growth under iron-restricted conditions resulted in the induction of several outer membrane proteins. At least four proteins, ranging in size from 50 kDa up to approximately 90 kDa, can be identified.