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United States Department of Agriculture

Agricultural Research Service

Title: Culture Media Design for Fermentation Bioprocesses

Author
item JACKSON, MARK

Submitted to: United States of America and Mexico Symposium
Publication Type: Abstract Only
Publication Acceptance Date: October 18, 2002
Publication Date: N/A

Technical Abstract: Target products for fermentation processes vary from whole cells to extracellular secondary metabolites. For all these processes, appropriate nutritional environments must be created and maintained to ensure optimized yields and product quality. Culture media optimization is a critical step in fermentation process development and often continues throughout the production life of the fermentation product. For many fermentation processes, two phases are involved: the biomass accumulation phase and product formation phase. Nutritional requirements must be optimized for the organism being grown and for the fermentation product desired. Since carbon, nitrogen, and oxygen represent the most abundant elemental components of the cell, their form, concentrations, and relative availability are often critical in culture media design. This talk will outline nutritional components that must be considered in culture media development and present appropriate experimental approaches for conducting these optimization studies. To demonstrate how these approaches can be used for culture media design, results from our studies on media optimization for microbial biocontrol agents and for fusarin C, a fungal secondary metabolite produced by Fusarium moniliforme, will be presented.

Last Modified: 9/10/2014
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