ARS | Publication request: RAPD ANALYSIS ON GENETIC DIVERSITY OF NIGHTSHADE SPECIES IN THE NORTH CENTRAL REGION

Submitted to: North Central Weed Science Society US Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: December 13, 2004
Citation: Sharkhuu, A., Goldsbrough, P.B., Goodwin, S.B., Weller, S.C. 2004. Rapd analysis on genetic diversity of nightshade species in the north central region. Proceedings of the 59th North Central Weed Science Society Meetings, December 13-16, 2004, Columbus, OH. p. 58.

Technical Abstract: The genetic diversity of eastern black nightshade species in the North Central (NC) U.S. (Michigan, Indiana, Illinois and Ohio) was analyzed by Random Amplification of Polymorphic DNA (RAPD) and compared to other solanaceous species from the NC and other areas of the U.S. including the annual nightshades horsenettle, American black nightshade and bitter nightshade, and perennials clammy groundcherry and smooth groundcherry. Twenty-five accessions of eastern black nightshade from various sites in the NC region were divided into 11 subgroups by cluster analysis based on the unweighted pair group method with arithmetic average. A single accession from each subgroup was selected randomly for further RAPD analysis with other solanaceous accessions collected throughout the U.S. In total, 232 RAPD markers were scored for presence or absence of strong reproducible bands. All accessions within a species had a similarity coefficient >0.75. Eastern black nightshade, black nightshade and American black nightshade were more genetically similar to each other than to other solanaceous species while bitter nightshade and ground cherry were distinct. Among the eastern black nightshade accessions studied, all but three were in the same cluster. Accessions of black nightshade, American black nightshade, horsenettle, hairy nightshade and bitter nightshade were each separated into distinct clusters except for two accessions that were originally considered to be black nightshade and horsenettle but through RAPD analysis were identified as American black nightshade and eastern black nightshade. These results confirm that RAPD analysis of genetic polymorphisms is a useful technique to characterize weedy solanaceous species.