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ARS Home » Pacific West Area » Albany, California » Plant Gene Expression Center » Research » Publications at this Location » Publication #173602
Title: TRANSFORMATION OF TOBACCO PLANTS WITH CDNA ENCODING HONEYBEE ROYAL JELLY MRJP1

Author
item JUDOVA, J. - SLOVAK REP ACAD SCIENCE
item SUTKA, R. - SLOVAK REP ACAD SCIENCE
item KLAUDINY, J. - SLOVAK REP ACAD SCIENCE
item LISKOVA, D. - SLOVAK REP ACAD SCIENCE
item Ow, David
item SIMUTH, J. - SLOVAK REP ACAD SCIENCE

Submitted to: Biologia Plantarum
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/19/2003
Publication Date: 12/1/2004
Citation: Judova, J., Sutka, R., Klaudiny, J., Liskova, D., Ow, D.W., Simuth, J. 2004. Transformation of tobacco plants with cDNA encoding honeybee royal jelly MRJP1. Biologia Plantarum, 48(2):185-191(7).

Interpretive Summary: This article describes the production of MRJP1 in tobacco, the major protein of honeybee royal jelly. The gene was incorporated into the tobacco genome and expressed from a constitutive promoter. The production of this component in plants might someday lead to synthetic substitutes of honeybee royal jelly that is used as a nutritional supplement.

Technical Abstract: For expression of MRJP1 - the most abundant protein of honeybee royal jelly - in plants, plasmid carrying the expression cassette composed of CaMV 35S RNA promoter, cDNA encoding MRJP1 with its native signal peptide, and nos3 as transcription terminator in binary vector pBin19 was prepared. The plasmid was introduced into tobacco (Nicotiana tabacum L. cv. Wi38) plants by Agrobacterium tumefaciens-mediated transformation. Transgenic F1 and F2 generation was grown from the seeds of the primary obtained transgenic tobacco plants. Immunoblot analyses of protein leaf extracts from transgenic plants showed expression of MRJP1.