ARS | Publication request: DEVELOPMENT OF GENOME-SPECIFIC STS MARKERS FOR PERENNIAL TRITICEAE GRASSES

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 2003
Publication Date: October 1, 2003
Citation: Wang, R., Lee, B.S., Mammadov, A. 2003. Development of genome-specific sts markers for perennial triticeae grasses. ASA-CSSA-SSSA Annual Meeting Abstracts.

Technical Abstract: Perennial Triticeae grasses serve as important gene pools for forages and cereal crops. Knowledge on their genome compositions is pivotal for efficient utilization of this vast gene pool in germplasm enhancement programs. Using DNA sequences of genome-specific RAPD markers, selected primers have been used to develop STS markers. Genome specificity was lost for a majority of RAPD-to-STS conversions due to the inward extension of primer sequences. However, successful conversion has been achieved for genomes Eb,Ee,St,Ns,W, and Y (an unknown in many polyploid Elymus species). Several CAPS markers were also developed to identify the R genome and to distinguish the St,Eb, and Ee genomes. Specificity of some non-targeted P-and H-genome STS markers need further validation tests. The identified STS and CAPS markers are useful in confirming the presence or absence of certain genome(s) in Triticeae species and in identifying the alien chromosome in wheat addition or substitution lines. Use of STS markers has helped identify wheat addition lines with W- and Y-genome chromosomes derived from hybrids of hexaploid wheat (AABBDD) and hexaploid Elymus rectisetus (StStYYWW). R-genome CAPS markers were used to identify the presence of translocation chromosome 1BL/1RS in the wheat background.