ARS | Publication request: SALT-STRESS TRANSCRIPT PROFILING OF SALT-TOLERANT WHEAT GERMPLASM

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2005
Publication Date: January 1, 2006
Citation: Mott, I.W., Chatterton, N.J., Wang, R. 2006. Salt-stress transcript profiling of salt-tolerant wheat germplasm. Plant and Animal Genome Conference.

Technical Abstract: Salt-tolerant wheat lines W4909 and W4910 were derived from a cross between AJDAj5 (a disomic addition line carrying a pair of Eb chromosomes from Thinopyrum junceum) and Ph1 (a line having the Ph1 allele from Aegilops speltoides). Both W4909 and W4910 have greater salt-tolerance than their parental lines, which are in turn more salt-tolerant than the common wheat background, Chinese Spring. AFLP analysis has shown that W4909 and W4910 contain 0.83% and 1.02% non-Chinese Spring markers, respectively, that are unique to either the AJDAj5 or Ph1 parent. Our aim in this study was to use the Wheat Genome ArrayTM to identify expression patterns in W4909 and W4910 that were attributable to either the AJDAj5 or Ph1 parent, and to identify transcripts that are induced under salt stress. Analysis of expression data identified 67 (0.11 % of total) and 93 (0.15% of total) probe sets that had parental expression levels that were traceable to W4909 and W4910, respectively. Of these probe sets in W4909, 36% came from AJDAj5 and 64% from Ph1. Of the probe sets in W4910, 13% came from AJDAj5 and 87% from Ph1. These results are comparable to those obtained by using AFLP, suggesting that the majority of introduced genetic material in these lines came from the Ph1 parent and a minority from AJDAj5. Additionally, 364 probe sets were induced or repressed between 4 to 619 fold in all five wheat lines under salt stress at EC=30 dS/m in leaf and root tissues.