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United States Department of Agriculture

Agricultural Research Service

Research Project: IPM TECHNOLOGIES FOR SUBTROPICAL INSECT PESTS

Location: Subtropical Insects and Horticulture Research

Title: Microsattelite Technology As a Tool for Managing Insecticide Resistance in Bemisia Tabaci (Biotypes B and Q)

Authors
item McKenzie, Cindy
item Boykin, Laura
item Byrne, Frank - UNIV. OF CA RIVERSIDE
item Bethke, Jim - UNIV. OF CA RIVERSIDE
item Shatters, Robert

Submitted to: National IPM Symposium
Publication Type: Abstract Only
Publication Acceptance Date: December 23, 2005
Publication Date: April 4, 2006
Citation: McKenzie, C.L., Boykin, L.M., Byrne, F., Bethke, J., Shatters, R.G. 2006. Microsattelite technology as a tool for managing insecticide resistance in Bemisia tabaci (Biotypes B and A). 5th National Integrated Pest Management Symposium Program and Abstracts. St. Louis, MO. p. 68-69 (PO35).

Technical Abstract: A new strain of Bemisia tabaci, “Q” biotype, was first detected in the U.S. on poinsettias purchased at a retail outlet during December 2004 in Tucson, Arizona. Although indistinguishable in appearance from silverleaf whitefly (B biotype), these insects proved markedly less susceptible to insect growth regulators and many neonicotinoids leaving few insecticide options for control. To date (12/05) Q biotype has been detected in 19 states. Microsattelites, relatively short tandem repeats of di-, tr- or tetra- nucleotides randomly distributed throughout the genome, were used as markers to study relationships among the U.S. populations of Q. The number of repeats at a particular locus is highly polymorphic between individuals of the same species or biotype making this a very powerful tracking tool that can be used for genetic fingerprinting and paternity testing. This new technology allows you not only to detect the presence of a particular biotype, but also determine where that insect originated from and if it is a product of hybridization. This is an extremely powerful tool when taken a step further and used with toxicological data (insecticide profiles). For example, knowing that a whitefly population infesting a grower in upstate New York came from a grower from southern California whose insecticide profile has been extensively evaluated should allow solid predictions on how a particular insecticide will perform on that population a priori. Microsatellite data on the relationship of Q populations collected throughout the U.S. are presented. Comparisons with Q populations from the region of origin (Spain, Israel, Northern Africa) are provided showing the nature of the emerging spread in the U.S., and application of this data to insecticide resistance management are discussed.

Last Modified: 4/20/2014
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