ARS | Publication request: DEVELOPMENT OF MICROSATELLITE MARKERS IN AN ESTUARINE OPOSSUM SHRIMP, AMERICAMYSIS BAHIA (CRUSTACEA: MYSIDA)

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 7, 2005
Publication Date: January 14, 2006
Citation: Bagnall, R.A., Rosell, R.C., Boykin, L.M., McKenzie, C.L., Frohlich, D.R., Shatters, R.G. 2006. Development of microsatellite markers in an Estuarine opossum shrimp, Americamysis bahia (Crustacea: Mysida). Plant and Animal Genome Conference. San Diego, CA. January 14-18.

Technical Abstract: Americamysis bahia is a sensitive, and EPA approved, indicator of estuarine water quality. Genomic DNA, prepared from 200-300 whole adults, was cut and size selected for 400-900 nt. After addition of linkers and PCR enrichment, products were probed with biotinylated di- and tetra- nucleotide probes, (CA)16, and GATA)8. Streptavidin coated paramagnetic beads were then used to bind hybridized sequences and repetitive DNAs were isolated and concentrated after denaturation. After a second PCR enrichment, putative microsatellites were TA cloned into a P-Gem vector and positive transformants were picked from a blue/white selection system. Herein, we report microsatellite sequences from A. bahia that may be useful in characterizing populations along the N. American Gulf and Mexican coasts.