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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGIES AND TECHNIQUES FOR THE DETECTION OF RESIDUES, TOXINS, AND OTHER CHEMICALS IN FOODS Title: Analysis of Oxytetracycline in Surface Water Using a Portable Analyzer

Authors
item Chen, Guoying
item Qin, Feng - SHANHAI INST OF DRUG CONT

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: July 10, 2006
Publication Date: October 1, 2006
Citation: Chen, G., Qin, F. 2006. Analysis of oxytetracycline in surface water using a portable analyzer. Proceedings SPIE 6378, 6378W.

Technical Abstract: A huge amount of oxytetracycline (OTC) is used in aquaculture worldwide. Since it is poorly absorbed by the gastrointestinal system and has a long lifetime, it is an important task to monitor its distribution and fate in the marine environment and its impact on human and the biosphere. A portable LED-based analyzer was prototyped employing europium-sensitized luminescence to perform field analysis of OTC in water, sediment, and other marine environmental matrices. In this study a simple and sensitive method for OTC analysis in surface water is developed using this instrument. OTC is first extracted and enriched from water onto 17×15 mm C18 sorbent strips under 1-hr constant stirring. The strips are then dipped into a Eu-containing reagent solution for 2 min. Next the dried strips are desiccated and exposed to a pulsed 385-nm UV beam. The time-resolved luminescence is directly measured on the sorbent surface. A 0-100 ppb linear dynamic range (LDR) (r2 = 0.999) and a 0.13-ppb limit of detection (LOD) are achieved with a typical 5% relative standard deviation (RSD). Based on the experimental results, this portable instrument and the assay method can be applied to aquacultural system monitoring, and to environmental analysis in general.

Last Modified: 10/30/2014
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