ARS | Publication request: DETECTION OF GENETIC VARIATION IN WILD POPULATIONS OF THREE ALLIUM SPECIES USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS (AFLP)

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: May 15, 2006
Publication Date: July 15, 2006
Citation: Phillips, N.C., Larson, S.R., Drost, D.T. 2006. Detection of genetic variation in wild populations of three allium species using amplified fragment length polymorphisms (aflp). HortScience. 41(4), 967

Technical Abstract: The genus Allium is distributed worldwide and includes about 80 North American species, with at least 13 occurring in Utah. This study examines amplified fragment length polymorphism (AFLP) variation within and among wild populations of three Allium species varying in abundance and distribution, native to Utah; Allium acuminatum, A. brandegei, and A. passeyi. DNA was extracted from individuals collected in 12 wild populations (A. acuminatum: 5, A. passeyi: 4, and A. brandegei: 3) using DNeasy 96 Plant Protocol. AFLP techniques were used to investigate the genetic diversity and population structure found within and among these populations. GeneScan software is being used to visually analyze for the presence and absence of DNA fragments between 50 and 400 base pairs. Genetic distances between individual plants will be determined, and pairwise comparisons computed using SAS.