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ARS Home » Southeast Area » Poplarville, Mississippi » Southern Horticultural Research Unit » Research » Publications at this Location » Publication #209301
Title: A New Electrophoresis Technique to Seperate Microsatellite Alleles

Author
item WANG, XINGWANG - UNIV OF TENN
item Rinehart, Timothy - Tim
item WADHL, PHILLIP - UNIV OF TENN
item Spiers, James
item JOHNSON, DENITA - UNIV OF TENN
item TRIGIANO, ROBERT - UNIV OF TENN

Submitted to: African Journal of Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/20/2008
Publication Date: 6/3/2009
Citation: Wang, X., Rinehart, T.A., Wadhl, P.A., Spiers, J.M., Johnson, D., Trigiano, R.N. 2009. A New Electrophoresis Technique to Seperate Microsatellite Alleles Vol.8 (11), pp.2432-2436. African Journal of Biotechnology.

Interpretive Summary: Traditional agarose and polyacrylamide gel electrophoresis have been used commonly for microsatellite (simple sequence repeats, SSRs) analysis, but they are labor- intensive and not always able to provide accurate sizes for different alleles. Capillary sequencers provide automated analysis and accurate allele sizes; however, the costs of the instrument, reagents and labeled primers make the use of sequencers uneconomical for most routine microsatellite analysis. Here, we report a fast, cost-effective and accurate method for doing routine microsatellite array analysis using a compact, bench-top multi-capillary electrophoresis system, the HDA-GT12 TM Genetic Analyzer (eGene, Irvine, CA, USA). SSRs from flowering dogwood (Cornus florida L.) were used to compare the different types of electrophoresis.

Technical Abstract: Traditional agarose and polyacrylamide gel electrophoresis have been used commonly for microsatellite (simple sequence repeats, SSRs) analysis, but they are labor- intensive and not always able to provide accurate sizes for different alleles. Capillary sequencers provide automated analysis and accurate allele sizes; however, the costs of the instrument, reagents and labeled primers make the use of sequencers uneconomical for most routine microsatellite analysis. Here, we report a fast, cost-effective and accurate method for doing routine microsatellite array analysis using a compact, bench-top multi-capillary electrophoresis system, the HDA-GT12 TM Genetic Analyzer (eGene, Irvine, CA, USA). SSRs from flowering dogwood (Cornus florida L.) were used to compare the different types of electrophoresis.