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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #221014
Title: Marker-assisted selection for powdery mildew in pea

Author
item McPhee, Kevin
item GHAFOOR, A. - NATL AG RES CNTR PAKISTAN

Submitted to: European Conference on Grain Legumes Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/5/2007
Publication Date: 10/5/2007
Citation: Mcphee, K.E., Ghafoor, A. 2007. Marker-assisted selection for powdery mildew in pea. European Conference on Grain Legumes Proceedings. pg 152

Interpretive Summary:

Technical Abstract: Powdery mildew, caused by Erysiphe pisi Syd. is an important pathogen of pea (Pisum sativum L.) worldwide. It causes significant yield loss and reduced crop quality when present in epidemic proportion. Genetic resistance is controlled by two recessive alleles, er-1 and er-2. Availability of co-dominant molecular markers closely linked to er-1 make marker assisted selection (MAS) a viable option for breeders. Seventeen populations from the USDA-ARS pea breeding program were selected to evaluate the utility of molecular markers for selection of resistant segregants. Up to 30 individuals from each population were grown under greenhouse conditions for DNA extraction and seed increase. Genotyping of the progeny was accomplished using 12 SSR markers flanking er-1 on pea LG VI. Progeny rows will be screened in the field for resistance and phenotypic data will be correlated with the molecular marker data. Application of molecular markers has potential to improve breeding efficiency; however, specific knowledge of the parental genotype or marker phase must be known prior to selection.