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United States Department of Agriculture

Agricultural Research Service

Title: A simple, bead-based assay for multiplex SNP analysis in wheat

Authors
item Kota, Raja - NORTH CAROLINA STATE UNIV
item Hall, Marla - VIRGINIA TECH
item Griffey, Carl - VIRGINIA TECH
item Brown-Guedira, Gina

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 20, 2007
Publication Date: December 20, 2007
Citation: Kota, R., Hall, M., Griffey, C., Brown Guedira, G.L. 2007. A simple, bead-based assay for multiplex SNP analysis in wheat. Meeting Abstract.

Technical Abstract: Single nucleotide polymorphisms (SNPs) are the most abundant form of DNA polymorphism and are highly suitable for automated analysis. These polymorphisms can be used in plants as simple genetic markers for many breeding applications and are useful for cultivar identification, genetic mapping, trait association and marker-assisted selection (MAS). With the influx of various SNP genotyping assays in recent years, there has been a need for an assay that is robust, yet cost effective, and suited toMAS. The Luminex system is a simple bead-based assay which utilizes a fluorescent microsphere sorter and can be used for low to medium throughput genotyping projects, capable of analyzing anywhere from one SNP in one individual up to 100 SNPs in unlimited numbers of individuals. This device uses FlexMAP beads that are fluorescent microspheres comprising 100 unique color codes enabling individual beads to be identified using a Luminex 100 instrument. This instrument is capable of analyzing either single-base extension (SBE), allele-specific primer extension (ASPE) or direct hybridization (DH) assays. Here, we present preliminary data on mapping SNPs in wheat and its potential uses in developing a high-throughput system for analyzing multiple SNPs in any given assay.

Last Modified: 7/31/2014
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