GENOMIC AND IMMUNOLOGICAL ANALYSIS OF SPIROCHETE DISEASES
Location: Infectious Bacterial Diseases Research Unit
Title: Genetic Diversity of the Leptospiral Immunoglobulin-like (Lig) Genes in Pathogenic Leptospira spp.
| Mcbride, Alan - FUNDACAO OSWALDO CRUZ |
| Cerqueira, Gustavo - FEDERAL UNIV. OF PELOTAS |
| Suchard, Marc - UCLA |
| Moreira, Angela - FEDERAL UNIV. OF PELOTAS |
| Zuerner, Richard |
| Reis, Mitermayer - FUNDACAO OSWALDO CRUZ |
| Haake, David - FUNDACAO OSWALDO CRUZ |
| Ko, Albert - CORNELL UNIV. |
| Dellagostin, Odir - FEDERAL UNIV. OF PELOTAS |
Submitted to: Infection, Genetics and Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 28, 2008
Publication Date: March 1, 2009
Citation: Mcbride, A.J., Cerqueira, G.M., Suchard, M.A., Moreira, A.N., Zuerner, R.L., Reis, M.G., Haake, D.A., Ko, A.I., Dellagostin, O.A. 2009. Genetic Diversity of the Leptospiral Immunoglobulin-like (Lig) Genes in Pathogenic Leptospira spp.. Infection, Genetics and Evolution. 9(2):196-205.
Interpretive Summary: Pathogenic Leptospira are the agents of leptospirosis, which is currently considered the most widespread zoonosis in the world. The focus of this paper was analysis of proteins belonging to a family of leptospiral immunoglobulin-like (Lig) proteins. These proteins appear to be promising candidates for vaccine and diagnostic assay development. We show that several types of evolutionary mechanisms have been acting on the genes directing synthesis of Lig proteins to generate diversity. This information is important for development of effective Lig-based vaccines.
Recent serologic, immunoprotection, and pathogenesis studies implicate the Lig proteins as key virulence determinants in interactions of leptospiral pathogens with the mammalian host. We examined the sequence variation and recombination patterns of ligA, ligB, and ligC among 10 pathogenic strains. Mean DNA and amino acid sequence variation was 8.4% and 11.1% for ligA, 19.4% and 21.8% for ligB, and 9.1% and 6.3% for ligC, respectively. All strains were found to have intact ligB genes and genetic drift accounted for most of the ligB genetic diversity. Like ligB, ligC gene variation also followed phylogenetic patterns, suggesting an early gene duplication event. However, several strains have inactivated versions of the ligC gene, suggesting that LigC is not essential for virulence. The ligA gene was found exclusively in L. interrogans and L. kirschneri strains, and appears to have been created from ligB by a two-step partial gene duplication process. Two ligB genes and one ligC gene had mosaic compositions. Evidence for recombination events between related bacterial species was also found for some ligA genes. In conclusion, the results presented here indicate that several types of evolutionary mechanisms have been acting on the lig genes, including genetic drift, gene duplication, and horizontal gene transfer.