Technical Abstract: Consumer awareness, pesticide and fertilizer contaminations, and environmental concerns have resulted in increased demand for organically grown farm products. The present study evaluates the influence that organic versus conventional farming practices exert on the total phenolic content in eggplant samples belonging to two cultivars, Blackbell (American eggplant) and Millionaire (Japanese eggplant) grown by conventional and organic farming practices with similar climatic conditions. In addition, plant-to-plant variation in phenolic content was also investigated with eight different eggplant samples collected from different plants grown at various field positions that were collected and separately assayed for phenolic content by two different assay procedures---HPLC/LC-MS and the Folin-Ciocalteu assay. The results of the HPLC analysis showed significant plant-to-plant variation (% RSD ranged from 22% to 48%) in the 8 eggplant samples collected from 8 different plants belonging to the same cultivar and grown under similar conditions. The Millionaire variety showed higher phenolic content as compared to the Blackbell variety. No significantly consistent trend in the phenolic content of eggplant samples grown with organic versus conventional farming practices was observed with either eggplant variety. A strong correlation (R2 = 0.87) between total phenolic content as assayed by the Folin-Ciocalteu assay and 5-caffeoylqunic acid, a major phenolic acid in eggplant extract, was observed.