ARS | Publication request: Characterization and Regulation of Suppressor of Cytokine Signaling (SOCS) Genes in Yellow Perch (Perca flavescens)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 25, 2009
Publication Date: March 1, 2010
Citation: Shepherd, B.S., Rees, C.B., Goetz, F.W. 2010. Characterization and Regulation of Suppressor of Cytokine Signaling (SOCS) Genes in Yellow Perch (Perca flavescens) [abstract]. In: 2010 World Aquaculture Society Meeting, March 1-5, 2010, San Diego, California. 2010. CDROM (Abstract 909).

Technical Abstract: The suppressor of cytokine signaling (SOCS) proteins are a family of intracellular proteins that are centrally involved with vertebrate growth, development, and immunity via their effects as negative feedback regulators of cytokine (and hormone) signaling. A number of SOCS genes have been recently characterized in finfish, but little is known of their involvement in the innate immune response, or of their hormonal control in commercially important teleosts. The genes for SOCS-1 & -3 were cloned, sequences analyzed, and expression patterns examined in the commercially important teleost, yellow perch (Perca flavescens). The deduced mature proteins for yellow perch SOCS-1 and SOCS-3 consist of 211 and 205 amino acids, respectively. Phylogenetic tree analysis showed that perch SOCS-1 & -3 share highest homology (among similar teleost sequences), to the stickleback (Gasterosteus aculatus) SOCS-1 & -3 protein homologues. Male and female yellow perch were immunologically challenged with a single injection (10 micrograms/g body weight) of lipopolysaccharide (LPS: a bacterial cell wall component) and tissues (gill, head kidney, kidney, liver and spleen) were sampled over a 48-hour time-course. RNA was extracted from tissues, and mRNA levels for SOCS-1 & -3 were quantified using quantitative real-time PCR (QPCR).