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ARS Home » Southeast Area » Little Rock, Arkansas » Microbiome and Metabolism Research Unit » Research » Publications at this Location » Publication #270194
Title: Biflavonoids from caper (capparis spinosa l.) Fruits and their effects in inhibiting nf-kappa b activation

Author
item ZHOU, HAI-FENG - Shanghai Institute Of Pharmaceutical Industry
item XIE, CHENGHUI - Arkansas Children'S Nutrition Research Center (ACNC)
item JIAN, RENJI - Shanghai Institute Of Pharmaceutical Industry
item KANG, JIE - Arkansas Children'S Nutrition Research Center (ACNC)
item LI, YAN - Shanghai Institute Of Pharmaceutical Industry
item ZHUANG, CHANG-LONG - Shanghai Institute Of Pharmaceutical Industry
item YANG, FANG - Shanghai Institute Of Pharmaceutical Industry
item LAI, LONG - Shanghai Institute Of Pharmaceutical Industry
item WU, TONG - Shanghai Institute Of Pharmaceutical Industry
item WU, XIANLI - Arkansas Children'S Nutrition Research Center (ACNC)

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/16/2011
Publication Date: 3/7/2011
Citation: Zhou, H., Xie, C., Jian, R., Kang, J., Li, Y., Zhuang, C., Yang, F., Lai, L., Wu, T., Wu, X. 2011. Biflavonoids from caper (capparis spinosa l.) Fruits and their effects in inhibiting nf-kappa b activation. Journal of Agricultural and Food Chemistry. 59(7):3060-3065.

Interpretive Summary: Caper (Capparis spinosa L.) fruits have been widely used as food and folk medicine in the Mediterranean basin and in central and west Asia. In this study, two biflavonoids, isoginkgetin, and ginkgetin, together with three other flavonoids, were isolated from caper fruits. To our knowledge, isoginkgetin, ginkgetin and sakuranetin were identified in caper for the first time. Concentrations of the two biflavonoids were measured in caper fruits collected from four major growing areas in northwest China. The anti-inflammatory effects of the flavonoids from caper fruits were evaluated by secreted placental alkaline phosphatase (SEAP) reporter assay. Isoginkgetin and ginkgetin showed inhibitory effects in initial screen at 20 µM, while the effect of ginkgetin was much greater than that of isoginkgetin.

Technical Abstract: Caper (Capparis spinosa L.) fruits have been widely used as food and folk medicine in the Mediterranean basin and in central and west Asia. In this study, two biflavonoids, isoginkgetin, and ginkgetin, together with three other flavonoids, were isolated from caper fruits. Their chemical structures were elucidated by spectroscopic analyses and comparison with literature. To our knowledge, isoginkgetin, ginkgetin and sakuranetin were identified in caper for the first time. Notably, it is also the first time that biflavonoids have ever been found in the Capparidaceae. Concentrations of the two biflavonoids were measured in caper fruits collected from four major growing areas in northwest China. The anti-inflammatory effects of the flavonoids from caper fruits were evaluated by secreted placental alkaline phosphatase (SEAP) reporter assay, which was designed to measure nuclear factor-kappa B (NF-'B) activation. Isoginkgetin and ginkgetin showed inhibitory effects in initial screen at 20 µM, while the effect of ginkgetin was much greater than that of isoginkgetin. In a dose-response experiment, the IC50 value of ginkgetin was estimated at 7.5 µM, suggesting it could be a strong NF-'B inhibitor and worthy of study in vivo.