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United States Department of Agriculture

Agricultural Research Service

Research Project: DISCOVERY AND UTILIZATION OF BIOACTIVE COMPONENTS FROM NEW CROPS AND AGRICULTURAL CO-PRODUCTS

Location: Functional Foods Research Unit

Title: Chlorogenic acid levels in leaves of coffee plants supplied with silicon and infected by Hemileia vastatrix

Authors
item Rodrigues, Fabricio -
item Carre-Missio, Vivian -
item Jham, Gulab -
item Berhow, Mark
item Schurt, Daniel -

Submitted to: Tropical Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 28, 2011
Publication Date: December 30, 2011
Citation: Rodrigues, F.A., Carre-Missio, V., Jham, G.N., Berhow, M.A., Schurt, D.A. 2011. Chlorogenic acid levels in leaves of coffee plants supplied with silicon and infected by Hemileia vastatrix. Tropical Plant Pathology. 36:404-408.

Interpretive Summary: Rust, caused by Hemileia vastatrix, is the main disease that decreases coffee production in Brazil. New and enhanced methods to reduce rust intensity that can be integrated with modern genetic and chemical approaches need to be investigated. Many plant species supplied with silicon show increased resistance to several pathogens, this study examined the possible effect of this element in increasing chlorogenic acid (CA) concentrations in coffee leaves and, consequently, increasing the level of resistance to rust. However, regardless of the increase in the concentrations of chlorogenic acid in leaves, coffee resistance to H. vastatrix infection was not potentialized by Si. This study was needed to confirm this observation.

Technical Abstract: Rust, caused by Hemileia vastatrix, is the main disease that decreases coffee production in Brazil. New and enhanced methods to reduce rust intensity that can be integrated with modern genetic and chemical approaches need to be investigated. Considering that many plant species supplied with silicon (Si) show increased resistance to several pathogens, this study examined the possible effect of this element in increasing chlorogenic acid (CA) concentrations in coffee leaves and, consequently, increasing the level of resistance to rust. Plants (cv. “Catuaí Vermelho IAC 44”) were inoculated with H. vastatrix after growing for 35 days in a hydroponic culture amended with 0 (-Si) or 2 (+Si) mM Si. Concentration of Si in leaf tissues was of 0.36 and 0.42 dag/kg for -Si and +Si treatments, respectively, but without a statistically significant difference. The area under rust progress curve was 154.5 and 119.4 for -Si and +Si treatments, respectively, but without significant statistical difference. For non-inoculated plants, the concentrations of total CA and caffeoyl-quinic acid (CQA) compounds (diCQA) were similar between -Si and +Si treatments. Even though there was an increase of 236.4 and 257.1%, respectively, for total CA and diCQA for +Si when compared to -Si treatment at 30 days after inoculation with H. vastatrix, reduction on rust severity was not obtained once the fungus had already colonized the leaf tissues. Therefore, regardless of the increase in the concentrations of chlorogenic acid on leaves, coffee resistance to H. vastatrix infection was not potentialized by Si.

Last Modified: 10/24/2014
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