ARS | Publication request: Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection

Submitted to: Microbiology and Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 22, 2013
Publication Date: April 1, 2013
Citation: Waters, S., Luther, S., Joerger, T., Richards, G.P., Boyd, E., Parent, M.A. 2013. Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection. Microbiology and Immunology. 57(4):323-328.

Interpretive Summary: Vibrio parahaemolyticus O3:K6 is a naturally occurring marine bacterium which was first isolated in India in 1996 and has spread to cause seafood-related illnesses throughout the world. This paper describes the response of mouse cells (macrophages) to V. parahaemolyticus O3:K6 infection, in order to provide a better idea of the mechanisms required to eliminate human infection. lammation. We found that there was significant changes in toll-like receptors on the surface of the macrophage. The data presented here will allow scientists to better understand the human response to V. parahaemolyticus infection and facilitate research to treat infection of fish and shellfish consumers.

Technical Abstract: Vibrio parahaemolyticus is the most common cause of bacterial seafood-related illness in the United States. Currently, there is a dearth of literature regarding immunity to infection with this pathogen. Here we studied V. parahaemolyticus-infected RAW 264.7 murine macrophage detecting both pro- and anti-inflammatory cytokine production, characterized by increased expression of IL-1 alpha, IL-6, TNF alpha and IL-10. Additionally, we discovered decreases in cell surface TLR2 and TLR4 while detecting increases in T cell co-stimulatory molecules CD40 and CD86. The data presented here begins to identify immune parameters required to eliminate V. parahaemolyticus from infected host tissues.