Technical Abstract: Phytophthora root and stem rot (PRR) disease, caused by P. sojae, is a widespread soybean disease resulting in an annual yield loss of $1~2 billion worldwide. To control the disease, breeders primarily employ race-specific resistant genes which are named Rps genes which have been identified to be located at 8 loci with 14 dominant alleles. However, soybean breeders are continuously facing big challenges due to the scarcity of excellent resistant genes and lack of efficient molecular markers for Marker Assisted Selection (MAS). Recently a soybean landrace PI567139B has been identified to confer excellent resistance to all major P.sojae races in Indiana soybean field and could provide potentially new resources of Rps genes. Genetic analysis revealed two dominant resistance genes and the resistance spectrum of each gene was characterized. Our results suggested that both of them were novel Rps genes.