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Research Project: GENETIC IMPROVEMENT OF CITRUS

Location: Subtropical Insects and Horticulture Research

Title: Conventional and transgenic resistance/tolerance to Huanglongbing in citrus

Authors

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 2012
Publication Date: N/A

Technical Abstract: Huanglongbing (HLB) is severely impacting Florida citrus, and has been found in California and Texas. Citrus researchers are immersed in extensive and broad-ranging efforts to identify solutions to HLB. Previous research indicates susceptibility to HLB throughout cultivated citrus: in FL none are immune and many are extremely adversely affected. Numerous transgenic strategies are underway to develop HLB/psyllid resistance in established cultivars, some show promise, and new ideas are added regularly. With HLB widespread in Florida, it is clear that not all cultivars are affected equally. HLB was assessed in commercial groves with high HLB-incidence: Temple had the mildest HLB symptoms and lowest Liberibacter (Las) titer, while Murcott and Minneola had the most severe symptoms and highest titer. The USDA Fort Pierce farm is managed to reveal genotype HLB responses. Some current cultivars and conventional hybrid seedlings demonstrate resistance/tolerance, at least to strain(s) of Las present. Some have abundant foliage symptoms, but full canopies and seemingly normal fruit set and size. For example, in a 3-year replicated trial of the grapefruit types Triumph(T), Jackson(J), Flame(F), and Marsh(M), all trees had HLB symptoms and similar Liberibacter titers. However T&J maintained full canopies and had fruit with normal size, yield and quality while F&M fruit were fewer and unacceptable. C. trifoliata is the best documented citrus resistance source, and its hybrids are being evaluated, with some already showing near-commercial fruit quality. Useful resistance/tolerance to HLB is present in cultivated citrus and more distant relatives, while transgenic methods offer tremendous potential for greater resistance. All are being investigated by the USDA citrus breeding program and collaborators, as well as other researchers.

   

 
Project Team
Bowman, Kim
Niedz, Randall
Stover, Ed
 
Publications
   Publications
 
Related National Programs
  Plant Genetic Resources, Genomics and Genetic Improvement (301)
  Plant Diseases (303)
 
Related Projects
   ANTIMICROBIAL PEPTIDE EVALUATION FOR HUANGLONGBING, CANKER AND PSYLLID RESISTANCE
   EVALUATION OF HLB/ACP RESISTANCE IN PONCIRUS TRIFOLIATA HYBRIDS AND ASSESSMENT OF DNA MARKERS AND POSSIBLE RESISTANCE GENES
   EVALUATION OF MYB1A GENE EXPRESSION IN CITRUS
   DOORYARD FRUIT CROPS FOR CENTRAL FLORIDA AS AN ALTERNATIVE TO CITRUS TO REDUCE REFUGE FOR ASIAN CITRUS PSYLLID
   EXPLORING AVOCADO VARIABILITY FOR LAUREL WILT RESISTANCE AND OTHER FRUIT QUALITY AND HORTICULTURAL TRAITS FOR PRODUCTION IN EAST-CENTRAL FL
   USDA-ARS CITRUS BREEDING TRUST WITH NEW VARIETIES DEVELOPMENT AND MANAGEMENT CORPORATION
   FOUNDER LINES FOR IMPROVED CITRUS BIOTECHNOLOGY
   NFCA BETWEEN USDA-ARS AND UNIVERSITY OF FLORIDA, IFAS AND FAES
   DEVELOPMENT OF PROMISING NEW SCIONS FOR FLORIDA CITRUS: EXPLOITING HUANGLONGBING (HLB) RESISTANCE AND TOLERANCE
   DEVELOPMENT OF PROMISING SUPERSOUR AND OTHER ROOTSTOCKS RESISTANT TO HUANGLONGBING
   HIGH-THROUGHPUT SCREENING OF TRANSGENIC CITRUS FOR HUANGLUNGBING (HLB) RESISTANCE
   PRODUCTION OF TRANSGENIC COMMERCIAL SCION CULTIVARS RESISTANT TO HLB AND CANKER: CONTINUED AMP APPROACHES AND NOVEL TRANSGENIC STRATEGIES
   ANALYZING LIBERIBACTER ISOLATES UNDETECTABLE BY STANDARD DIAGNOSTIC METHODS IN FLORIDA
   Development of promising supersour and other rootstocks resistant to huanglonging (HLB)
   Development of promising supersour and other rootstocks resistant to HLB (Huanglongbing)
   Identification and characterization of HLB-induced small RNAs and mRNAs-towards the understanding of natural defense mechanisms against HLB
   INVESTIGATING MECHANISMS OF PATHOGENESIS AND NATURAL DEFENSE RESPONSES OF CITRUS GREENING (HLB) BY CHARACTERIZING HOST SMALL RNAS AND MRNAS
   SECURE SITE FOR TESTING TRANSGENIC AND CONVENTIONAL CITRUS FOR HLB AND PSYLLID RESISTANCE
 
 
Last Modified: 05/25/2013
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