Technical Abstract: Closely related novel Wolbachia strains were recovered from the thelytokous figitids Odontosema anastrephae Borgmeier and Aganaspis alujai Ovruski et al. No Wolbachia were detected in a bi-sexual strain of O. anastrephae. While the presence or absence of Wolbachia does not demonstrate that Wolbachia is responsible for the lack of males produced by infected females, multilocus sequence typing failed to identify other endosymbionts that might have caused sex-ratio distortions. The phylogenies of insects and their Wolbachia are often not parallel and closely related bacteria infecting apparently more distantly related hosts suggests a horizontal transfer occurred after the divergence of the wasps. Given the figitids present ecology there are few obvious opportunities for transfer through host or habitat-sharing.