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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND BIOLOGICAL DETERMINANTS OF AVIAN TUMOR VIRUS PATHOGENICITY, TRANSMISSION, AND EVOLUTION

Location: Avian Disease and Oncology Laboratory

Title: Insertion of reticuloendotheliosis virus long terminal repeat into the genome of CVI988 strain of Marek’s disease virus results in enhanced growth and protection

Authors
item Lupiani, Blanca -
item Lee, Lucy
item Kreager, Ken -
item Witter, Richard
item Reddy, Sanjay -

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 28, 2012
Publication Date: January 14, 2013
Citation: Lupiani, B., Lee, L.F., Kreager, K.S., Witter, R.L., Reddy, S.M. 2013. Insertion of reticuloendotheliosis virus long terminal repeat into the genome of CVI988 strain of Marek’s disease virus results in enhanced growth and protection. Avian Diseases. 57(2):427-431.

Interpretive Summary: Mark’s disease (MD), a virus-induced cancer-like disease of chickens, is a major disease problem in commercial poultry. Vaccination of commercial poultry has drastically reduced losses from MD and the poultry industry cannot be sustained without the use of vaccines. The objective of this research was to develop and improve vaccines. We have developed a novel recombinant MD virus (MDV) vaccine named CVRM. This virus replicated to higher levels than parental CVI988 in cell culture and remained apathogenic for chickens. In addition, CVRM showed protection indices similar or superior to those afforded by CVI988 virus in laboratory and field protection trials, suggesting it could be developed as a safe and efficacious vaccine to protect against very virulent plus MDV. This information is of great importance to the poultry industry as vaccine companies seek future vaccine for control of MD.

Technical Abstract: Marek’s disease (MD) is a lymphoproliferative disease of chicken caused by serotype 1 MD virus (MDV). Vaccination of commercial poultry has drastically reduced losses from MD and the poultry industry cannot be sustained without the use of vaccines. Retrovirus insertion into herpesviruses genome is an efficient process that alters the biological properties of herpesviruses. RM1, a virus derived from virulent JM strain of MDV by insertion of the Reticuloendotheliosis (REV) LTR was attenuated for oncogenicity but retains properties of the parental virus such as lymphoid organ atrophy. Here we show that insertion of the REV LTR into the genome of vaccine strain CVI988, resulted in a virus, CVRM, that replicated to higher levels than parental CVI988 in cell culture and remained apathogenic for chickens. In addition, CVRM showed protection indices similar or superior to those afforded by CVI988 virus in laboratory and field protection trials, suggesting it could be developed as a safe and efficacious vaccine to protect against very virulent plus MDV.

Last Modified: 9/10/2014
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