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United States Department of Agriculture

Agricultural Research Service

Research Project: PSEUDOMONAS SYSTEMS BIOLOGY

Location: Plant-Microbe Interactions Research

Title: RecTEPsy mediated recombineering in Pseudomonas syringae

Author
item Swingle, Bryan

Submitted to: Methods in Molecular Biology
Publication Type: Other
Publication Acceptance Date: February 25, 2013
Publication Date: January 31, 2014
Citation: Swingle, B.M. 2014. RecTEPsy mediated recombineering in Pseudomonas syringae. Methods in Molecular Biology. 1114:3-10. DOI: 10.1007/978-1-62703-761-7_1.

Technical Abstract: A recently developed Pseudomonas syringae recombineering system simplifies the procedure for installing specific mutations at a chosen genomic locus. The procedure involves, transforming P. syringae cells expressing recombineering functions with a PCR product that contains desired changes flanked by sequences homologous to a target location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.

Last Modified: 12/19/2014
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