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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED PLANT GENETIC RESOURCES FOR PASTURES AND RANGELANDS IN THE TEMPERATE SEMIARID REGIONS OF THE WESTERN U.S. Title: Using DNA markers to distinguish among species and populations in seed lots

Authors
item Bushman, Shaun
item Larson, Steven
item Mott, Ivan

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 10, 2013
Publication Date: April 10, 2013
Citation: Bushman, B.S., Larson, S.R., Mott, I.W. 2013. Using DNA markers to distinguish among species and populations in seed lots. Native Seed Conference, Santa Fe, NM. Meeting Abstract.

Technical Abstract: Sometimes visual measurements are insufficient to distinguish seed contaminants in a seed lot, or native vs. introduced species of taxa that have overlapping morphological characters. Often visual measurements are insufficient to distinguish among different populations of a species. In each of these instances molecular markers can be used with great efficacy to distinguish between targeted plants. Molecular fingerpringing can target and utilize markers from chloroplast regions, expressed nuclear genes, or random genomic fragments. When developing markers for identity uses in a species, obtaining a set of unique markers is affected by the breeding system of plants and the use of appropriate checks or known standards. Chloroplast DNA markers have been used to differentiate native from exotic mannagrasses (Glyceria spp.) and proved that exotic and invasive species were inadvertently being used for revegetation of vernal pools. Markers from expressed genes have been used in numerous species to differentiate between cultivars and populations for identification purposes. We will show how molecular markers have been crucial in some plant identification situations, how they can be successfully used, and what their pragmatic limitations are.

Last Modified: 12/22/2014