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United States Department of Agriculture

Agricultural Research Service

Research Project: Improving Immunity, Health, and Well-Being in Cattle and Swine

Location: Livestock Issues Research

Title: Prenatal transportation alters the metabolic response of Brahman bull calves exposed to a lipopolysaccharide (LPS) challenge

Authors
item Carroll, Jeffery
item Sanchez, Nicole
item Roberts, Meghan -
item Price, Deborah -
item Littlejohn, Brittni -
item Vann, Rhonda -
item Welsh Jr, Thomas -
item Hughes, Heather -
item Richeson, John -
item Randel, Ronald -

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: March 12, 2013
Publication Date: October 24, 2013
Citation: Carroll, J.A., Sanchez, N.C., Roberts, M.C., Price, D.M., Littlejohn, B.P., Vann, R.C., Welsh Jr, T.H., Hughes, H.D., Richeson, J.T., Randel, R.D. 2013. Prenatal transportation alters the metabolic response of Brahman bull calves exposed to a lipopolysaccharide (LPS) challenge. Journal of Animal Science Supplement. 91(E-Suppl. 2): 413 (Abstract 371).

Technical Abstract: This study was designed to determine if prenatal transportation influences the metabolic response to a postnatal lipopolysaccharide (LPS) challenge. Pregnant Brahman cows (n=96) matched by age and parity were separated into transported (TRANS; n=48; transported for 2 hours on gestational day 60, 80, 100, 120 and 140) and non-transported control groups (CONT; n=48). From these treatments, bull calves (n=16 per treatment) were identified at weaning (176 +/- 2 d of age) to subsequently receive a LPS challenge. We previously reported an effect of TRANS on temperament (TEMP); therefore, bulls were also grouped based on TEMP score [Calm (C); Intermediate (I), or temperamental (T)]. On d -2, bulls were transported by trailer from Overton, TX, to Lubbock, TX, (~750 km, ~ 8.5 h). On day -1, bulls were fitted with indwelling jugular cannulas and placed in individual stalls. On day 0, blood samples were collected at 0.5-hour intervals from -2 to 8 hours, and again at 24 hours relative to LPS challenge (0.5 microgram/kg body weight) at Time 0. Serum was analyzed for glucose, insulin, non-esterified fatty acids (NEFA), and blood urea nitrogen (BUN) concentrations. All variables increased post-LPS (P<0.01). Glucose was greater in TRANS than CONT pre- and post-LPS (P<0.01), and was greater in T than C and I bulls pre-LPS (P<0.01). Post-LPS glucose was affected by TEMP, with this response influenced by prenatal transportation (P<0.01). Insulin tended to be greater (P=0.09) in TRANS than CONT bulls pre- and post-LPS. Post-LPS insulin was greatest in the C TRANS and I CONT bulls (P<0.01). Pre-LPS NEFA was greater in CONT than TRANS bulls (P=0.02), and was greatest in I bulls (P<0.01). Post-LPS, NEFA was also greater in I bulls (P<0.01). The BUN was greater both pre- and post-LPS in the C TRANS bulls. In summary, both prenatal transportation and TEMP had significant effects on the metabolic response before and after LPS, with prenatal transportation altering the observed metabolic responses within temperament groups. The altered metabolic response in the TRANS bulls may help explain differences observed in markers of the acute phase response in these bull calves following the LPS challenge.

Last Modified: 9/21/2014
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