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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION OF DISEASE MECHANISMS AND CONTROL STRATEGIES FOR BACTERIAL RESPIRATORY PATHOGENS IN CATTLE Title: Mycoplasma bovis research update

Authors
item Register, Karen
item Sacco, Randy
item Olsen, Steven

Submitted to: Trade Journal Publication
Publication Type: Trade Journal
Publication Acceptance Date: April 26, 2013
Publication Date: April 29, 2013
Repository URL: http://www.bisoncentral.com
Citation: Register, K.B., Sacco, R.E., Olsen, S.C. 2013. Mycoplasma bovis research update. Trade Journal Publication. Available: http://www.bisoncentral.com.

Interpretive Summary: Research conducted at the USDA/ARS/National Animal Disease Center in Ames, Iowa, reveals that ELISAs designed for detection of M. bovis-specific serum IgG in cattle may not be optimal for identification of seropositive bison, particularly those with low to moderate levels of antibody. In a study soon to be published, Drs. Karen Register, Randy Sacco and Steven Olsen report there is considerable variability in the performance of those assays with sera from bison immunized or infected with M. bovis. Their data demonstrate that some reagents used to detect cattle IgG are suboptimal for detection of bison IgG. They additionally report that higher sensitivity is achieved when the antigen used to capture anti-M. bovis antibody is sourced from bison isolates rather than cattle isolates, suggesting the specificity of the immune response to M. bovis may differ in bison as compared to cattle. These observations establish basic parameters of importance and provide a starting point for the development of a more sensitive, standardized enzyme immunoassay for identification of bison seropositive for M. bovis.

Technical Abstract: Research conducted at the USDA/ARS/National Animal Disease Center in Ames, Iowa, reveals that ELISAs designed for detection of M. bovis-specific serum IgG in cattle may not be optimal for identification of seropositive bison, particularly those with low to moderate levels of antibody. In a study soon to be published, Drs. Karen Register, Randy Sacco and Steven Olsen report there is considerable variability in the performance of those assays with sera from bison immunized or infected with M. bovis. Their data demonstrate that some reagents used to detect cattle IgG are suboptimal for detection of bison IgG. They additionally report that higher sensitivity is achieved when the antigen used to capture anti-M. bovis antibody is sourced from bison isolates rather than cattle isolates, suggesting the specificity of the immune response to M. bovis may differ in bison as compared to cattle. These observations establish basic parameters of importance and provide a starting point for the development of a more sensitive, standardized enzyme immunoassay for identification of bison seropositive for M. bovis.

Last Modified: 12/18/2014
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