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United States Department of Agriculture

Agricultural Research Service

Research Project: FINGERPRINTING AND PROFILING METHODS FOR CHARACTERIZATION OF FOODS AND DIETARY SUPPLEMENTS

Location: Food Composition and Methods Development Lab

Title: The effect of surfactant on headspace single drop microextraction for the determination of some volatile aroma compounds in citronella grass and lemongrass leaves by gas chromatography

Authors
item Jumepaeng, T -
item Luthria, Devanand
item Chanthai, S -

Submitted to: Analytical Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 18, 2011
Publication Date: April 20, 2012
Citation: Jumepaeng, T., Luthria, D.L., Chanthai, S. 2012. The effect of surfactant on headspace single drop microextraction for the determination of some volatile aroma compounds in citronella grass and lemongrass leaves by gas chromatography . Analytical Methods. 4:2012,424-28.

Interpretive Summary: A rapid method for the determination of some volatile aromatic compounds (VACs), including citronellal, citronellol, neral, geranial, geraniol and eugenol in citronella grass and lemongrass leaves, was developed using surfactant as a surface tension modifier while performing headspace single drop microextraction (HS-SDME) prior to analysis by GC-FID. T. Jumepaeng visited Food Composition Methods Development Laboratory for six months. Dr. S. Chanthai was his Research Advisor from University of Khon Kaen, Thailand.

Technical Abstract: A rapid method for the determination of some volatile aromatic compounds (VACs), including citronellal, citronellol, neral, geranial, geraniol, and eugenol in citronella grass and lemongrass leaves, was developed using surfactant as a surface tension modifier while performing headspace single drop microextraction (HS-SDME) prior to analysis by GC-FID. Experimental parameters were optimized,including type of single drop solvent, extraction temperature and time, enrichment time, sample volume, and salt and surfactant concentration. In this study, the effect of surfactants (SDS, Triton-X and CTAB) on the HS-SDME of VACs was investigated in detail. It was found that a small amount of SDS could contribute to decreasing the surface tension of liquid phase, resulting in an improvement of the extraction efficiency. The proposed method gave higher concentration factors (2.39–24.6 fold) of these VACs in real samples, and good recovery (97.6–109%) and precision (7–15% RSD). The advantage of this procedure is then recognized as a green chemistry because its simple and cheap method with reduced analysis time and toxicity. The sample preparation can easily be applied to routine analysis of these VACs in citronella grass and lemongrass leaf samples.

Last Modified: 7/25/2014
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