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United States Department of Agriculture

Agricultural Research Service

Research Project: Primary and Secondary Prevention of Peanut and Tree Nut Allergy

Location: Food Processing and Sensory Quality Research

Title: Structure and function of the peanut panallergen Ara h 8

Authors
item Hurlburt, Barry
item Offermann, Lesa -
item McBride, Jane
item Majorek, Karolina -
item Maleki, Soheila
item Chruszcz, Maksymilian -

Submitted to: Journal of Biological Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 18, 2013
Publication Date: February 11, 2014
Citation: Hurlburt, B.K., Offermann, L.R., Mcbride, J.K., Majorek, K.A., Maleki, S.J., Chruszcz, M. 2014. Structure and function of the peanut panallergen Ara h 8. Journal of Biological Chemistry. 288:36890-36901.

Interpretive Summary: The incidence of peanut allergy continues to rise in the U.S. and Europe; whereas, exposure to the major allergen proteins Ara h 1, 2, 3, and 6 can cause fatal anaphylaxis, exposure to the minor allergens usually does not. Ara h 8 is a minor allergen. Importantly, it is the minor food allergens that are thought to be responsible for Oral Allergy Syndrome (OAS), in which sensitization to airborne allergens causes an allergic reaction to ingested foods. In this work we determined the 3-dimensional structure of Ara h 8. We also found that Ara h 8 can bind hydrophobic molecules.

Technical Abstract: The incidence of peanut allergy continues to rise in the US and Europe; whereas, exposure to the major allergens Ara h 1, 2, 3, and 6 can cause fatal anaphylaxis, exposure to the minor allergens usually does not. Ara h 8 is a minor allergen. Importantly, it is the minor food allergens that are thought to be responsible for Oral Allergy Syndrome (OAS), in which sensitization to airborne allergens causes a Type 2 allergic reaction to ingested foods. Furthermore, it is believed that similar protein structure, rather than a similar linear sequence, is the cause of OAS. Bet v 1 from birch pollen is a common sensitizing agent, and OAS results when patients consume certain fruits, vegetables, tree nuts, and p\peanuts. Here, we report the 3-dimensional structure of Ara h 8, a Bet v 1 homolog. The overall fold is very similar to that of Bet v 1, Api g 1 (celery), Gly m 4 (soy), and Pru av 1 (cherry). Ara h 8 binds the isoflavones quercetin and apigenin, as well as resveratrol avidly.

Last Modified: 11/24/2014
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