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United States Department of Agriculture

Agricultural Research Service

Research Project: Development and Characterization of Soybean Germplasm, Curation of Stored Accessions, and Regional Evaluations of New Genotypes

Location: Crop Genetics Research Unit

Title: Quantitative trait loci for seed isoflavones contents in 'MD96-5722' by 'Spencer' recombinant inbred lines of soybean

Authors
item Akond, Masum -
item Liu, Shiming -
item Kantartzi, Stella -
item Mekem, Khalid -
item Bellaloui, Nacer
item Lightfoot, David -
item Yuan, Jiazheng -
item Wang, Dechun -
item Kassem, Abdelmajid -

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 29, 2014
Publication Date: January 29, 2014
Citation: Akond, M., Liu, S., Kantartzi, S.K., Mekem, K., Bellaloui, N., Lightfoot, D., Yuan, J., Wang, D., Kassem, A.M. 2014. Quantitative trait loci for seed isoflavones contents in 'MD96-5722' by 'Spencer' recombinant inbred lines of soybean. Journal of Agricultural and Food Chemistry. 62:1464-1468.

Interpretive Summary: Isoflavones are phytohormones (plant hormones) found in soybean seed and have beneficial effects on human nutrition and health. Therefore, breeding and selecting soybean for high isoflavones content is important. One possible way to do so is through what is called marker assisted selection (a genetic selection process that uses a DNA marker (indicator) to identify a trait characteristic, in our case isoflavones content in seed). Wide variations in seed content of isoflavones daidzein, glycitein, genistein, and total isoflavones were found among soybean populations studied. Three chromosomal regions were identified related to isoflavones. One region controlled isoflavone daidzein content and two regions controlled glycitein content. These identified regions for isoflavones can be used to develop soybean with preferred isoflavone concentrations in soybean seed.

Technical Abstract: Isoflavones from soybeans (Glycine max L. Merr.) have significant impact on human health in reducing the risk of several major diseases. Breeding soybean for high isoflavones content in the seed is possible through marker assisted selection (MAS), which can be based on quantitative trait loci (QTL). The objective of this study was to identify QTL controlling isoflavone content in a set of ‘MD96-5722’ by ‘Spencer’ recombinant inbred line (RIL) populations of soybean. Wide variations were found for seed concentrations of daidzein, glycitein, genistein, and total isoflavones among the RIL populations. Three QTL were identified on three different linkage groups (LG) represented by three different chromosomes (Chr.). One QTL that controlled daidzein content was identified on LG A1 (Chr 5) and two QTL that underlay glycitein content were identified on LG K (Chr_9) and LG B2 (Chr 14). Identified QTL could be functional to develop soybean with preferable isoflavones concentrations in the seeds of soybean through MAS.

Last Modified: 10/24/2014
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