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United States Department of Agriculture

Agricultural Research Service

Research Project: IPM TECHNOLOGIES FOR INSECT PESTS OF ORCHARD CROPS

Location: Subtropical Insects and Horticulture Research

Title: Asian citrus psyllid genome (Diaphorina citri, Hemiptera)-Update 2013

Authors
item Hunter, Wayne
item Reese, Justin -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 17, 2013
Publication Date: October 15, 2013
Citation: Hunter, W.B., Reese, J. 2013. Asian citrus psyllid genome (Diaphorina citri, Hemiptera)-Update 2013 [abstract], October 15-17, Denver, Colorado.

Technical Abstract: The psyllid genome is a scientific breakthrough that opens the psyllid genetic blueprint to investigations of all questions ranging from taxonomic origins to the understanding of developmental biology, to the acquisition and transmission of pathogens. The Asian citrus psyllid, Diaphorina citri (Hemiptera) threatens the citrus industry as a vector of the plant-infecting bacteria, Candidatus Liberibacter asiaticus, associated with the devastating disease, Huanglongbing. Psyllids are major disease vectors of many fruit tree crops yet their genetics have remained poorly studied. This genome draft, Diaphorina citri, DIACI_1.0 used additional sequencing from the PacBio system, Los Alamos National Lab, New Mexico, and new software PB-Jelly, to improve the number of resolved bases by over 10 million. The genome and transcriptome have been submitted to National Center for Biotechnology Information, NCBI,[http://www.ncbi.nlm.nih.gov/genome?LinkName=bioproject_genome&from_uid=29447] for access by the larger research community. The psyllid transcriptome has been refined down to about 18,400 predicted genes and the transcript and protein files can be downloaded at: www.psyllid.org/download, no password needed. These data are supported by an additional 19,598 EST’s produced previously for the psyllid hosted at NCBI (Hunter et al, 2005-2008). Life stage specific transcripts were identified for adults, nymphs and eggs. BlastX analyses showed the most similarity to the Pea Aphid, Acyrthosiphon pisum, another hemipteran. These data provide for RNAi development. Annotation of the psyllid genome will provide information on genome structure and psyllid biology.

Last Modified: 12/18/2014
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