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United States Department of Agriculture

Agricultural Research Service

Research Project: UNDERSTANDING THE ROLE OF COMMENSAL ANAEROBIC BACTERIA IN ODOR, EMISSIONS, AND ANTIBIOTIC RESISTANCE FROM STORED LIVESTOCK MANURE

Location: Bioenergy Research Unit

Title: Peptoniphilus stercorisuis sp. nov. from a swine manure storage tank and description of Peptoniphilaceae fam. nov.

Authors
item Johnson, Crystal -
item Whitehead, Terence
item Cotta, Michael
item Rhoades, Robert -
item Lawson, Paul -

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 21, 2014
Publication Date: N/A

Interpretive Summary: Odor emanating from stored manure present on swine production facilities has increased the tension among rural neighbors and among urban and rural residents. Storage of swine manure is associated with the production of a variety of odorous compounds, including ammonia, volatile organic acids and alcohols, and sulfides. In order to reduce production of odorous compounds, the responsible bacteria present in the fecal matter and manure storage pits must first be identified. We now report on the identification and scientific naming of a new species of anaerobic bacteria, Peptoniphilis oklahomaense. This species is similar to other bacteria capable of degrading proteins to ammonia and other odorous compounds. This information will be of use to other researchers and environmental agencies.

Technical Abstract: A species of a previously unknown Gram-positive, anaerobic, coccus-shaped bacterium recovered from swine faeces storage tanks was characterized using phenotypic, chemotaxonomic, and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and biochemical characteristics demonstrated that this organism is genotypically and phenotypically distinct, and represents a previously unknown sub-line within the order Clostridiales, within the Firmicutes. Pairwise sequence analysis demonstrated that the novel organism clustered within the genus Peptoniphilus, most closely related to Peptoniphilus methioninivorax sharing a16S ribosomal RNA gene sequence similarity of 95.5%. The major long-chain fatty acids were found to be C14:0 (22.4%) and C16:0 (15.6%). Based on the phenotypic and phylogenetic findings, a novel species Peptoniphilus oklahomaense sp. nov. is proposed. The type strain of Peptoniphilus oklahomaense sp. nov. is SF-S1T (DSM 27563T = NBRC 109839T).

Last Modified: 10/25/2014
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