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United States Department of Agriculture

Agricultural Research Service

Research Project: Improving Fruit Quality, Disease Resistance, and Tolerance to Abiotic Stress in Grape

Location: Grape Genetics Research

Title: VitisGen: A coordinated effort in grapevine genotyping, phenotyping, and marker assisted breeding.

Authors
item Reisch, Bruce -
item Cadle-Davidson, Lance
item Londo, Jason
item Takacs, Elizabeth -
item Hyma, Katie -
item Sun, Qi -
item Barba, Paola -
item Fennell, Anne -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 17, 2014
Publication Date: N/A

Technical Abstract: To enable early selection of elite seedlings combining disease resistance, stress tolerance, and fruit quality, U.S. grape breeders and geneticists developed a coordinated strategy for marker discovery and application using centralized phenotyping and map development via genotyping-by-sequencing (GBS). This 5-year project (“VitisGen”) began in 2011, and 7,000 breeding lines were genotyped in the first year to track alleles introgressed from twelve Vitis species. Our current GBS pipeline results in the mapping of over 20,000 SNPs per population using algorithms suitable for double pseudo-testcross populations. These dense genetic maps combined with the centralized phenotyping approach will lead to the development of at least 30 marker sets for alleles controlling traits of current and future interest for public grape breeding programs. Precision phenotyping is coordinated through separate centers focusing on powdery mildew resistance, low temperature response, and fruit quality. Breeders are phenotyping additional traits in 19 mapping populations with the goal of using all project phenotyping data to locate QTLs associated with a range of viticulturally important traits. The VitisGen project also provides breeders with annual access to marker-assisted selection procedures, initially focusing on previously published SSR markers for disease resistance, flower sex, and other traits.

Last Modified: 12/21/2014
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