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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Approaches for More Efficient Breeding to Improve Cotton Fiber Quality Traits

Location: Cotton Fiber Bioscience Research Unit

Title: Identification of cotton fiber quality quantitative trait loci using intraspecific crosses derived from two near-isogenic lines differing in fiber bundle strength.

Authors
item Islam, Md
item Zeng, Linghe
item Delhom, Christopher
item Song, Xianliang -
item Kim, Hee-Jin
item Li, Ping
item Fang, David

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 23, 2014
Publication Date: February 12, 2014
Citation: Islam, M.S., Zeng, L., Delhom, C.D., Song, X., Kim, H.J., Li, P., Fang, D.D. 2014. Identification of cotton fiber quality quantitative trait loci using intraspecific crosses derived from two near-isogenic lines differing in fiber bundle strength. Molecular Breeding. 34:373-384.

Interpretive Summary: Cotton fiber properties are very important to the yarn quality. Modern high-speed textile operations around the world require long, strong and fine cotton fibers. The objective of this research was to identify stable fiber quantitative trait loci (QTL) that could be used in cotton breeding through marker assisted selection (MAS). Two Upland cotton lines, MD90ne and MD52ne, are near isogenic (almost identical) with significant difference in fiber properties especially strength. Fiber samples from 734 progeny plants of two F2 populations (#1 & 5) derived from crosses between MD90ne and MD52ne were collected at Stoneville, MS in 2012. Fiber quality attributes were measured. A genetic linkage map with 165 loci covering 632.53 cM was constructed using the Population 5 consisting of 356 F2 individuals and used for identifying QTLs related to fiber bundle strength (FBS), short fiber index (SFI) and upper half mean length (UHML). One major QTL for FBS originated from the stronger fiber parent MD52ne was identified on chromosome (Chr.) 3. Three QTLs each for SFI and UHML were identified on Chr.3, 4, 14 and Chr.3, 11, 24, respectively. The Population 1 consisting of 378 F2 progeny was used to validate these QTLs by analyzing 57 SSR markers mapped on Chr.3, 14 and 24. Three QTLs, i.e. qFBS-c3, qSFI-c14, and qUHML-c24, were validated and appeared stable. These three QTLs could be potentially used in breeding to improve cotton fiber quality through a MAS strategy.

Technical Abstract: Cotton fiber properties are very important to the yarn quality. Modern high-speed textile operations around the world require long, strong and fine cotton fibers. The objective of this research was to identify stable fiber quantitative trait loci (QTL) that could be used in cotton breeding through marker assisted selection (MAS). Two Upland cotton lines, MD90ne and MD52ne, are near isogenic with significant difference in fiber properties especially strength. Fiber samples from 734 progeny plants of two F2 populations (#1 & 5) derived from crosses between MD90ne and MD52ne were collected at Stoneville, MS in 2012. Fiber quality attributes were measured using a High Volume Instrument 1000. A simple sequence repeat (SSR) genetic linkage map with 165 loci covering 632.53 cM was constructed using the Population 5 consisting of 356 F2 individuals and used for identifying QTLs related to fiber bundle strength (FBS), short fiber index (SFI) and upper half mean length (UHML). One major QTL for FBS originated from the stronger fiber parent MD52ne was identified on chromosome (Chr.) 3. Three QTLs each for SFI and UHML were identified on Chr.3, 4, 14 and Chr.3, 11, 24, respectively. The Population 1 consisting of 378 F2 progeny was used to validate these QTLs by analyzing 57 SSR markers mapped on Chr.3, 14 and 24. Three QTLs, i.e. qFBS-c3, qSFI-c14, and qUHML-c24, were validated and appeared stable. These three QTLs could be potentially used in breeding to improve cotton fiber quality through a MAS strategy.

Last Modified: 11/24/2014
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