Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 25, 2014
Publication Date: April 1, 2014
Citation: Neill, J.D., Ridpath, J.F. 2014. Complete genome sequence of a cytopathis/noncytopathis pair of border disease viruses. Genome Announcements. 2(2). doi: 10.1128/genomeA.00260-14. Interpretive Summary: Border disease virus (BDV) is a close relative of bovine viral diarrhea virus (BVDV). BDV is generally associated with infections of small ruminants, such as sheep and goats. Like BVDV, BDV consists of two biotypes, cytopathic and non-cytopathic, depending on the activity in cell culture. Here, the complete genome sequences of a cytopathic/non-cytopathic pair of BDV were determined. This is the first such pair of BDV to be completely sequenced. This showed that the cytopathic virus had a cellular insert of 285 bases in the NS2/NS3 region that gave it the cytopathic biotype. There were a total of 50 nucleotide differences between the two viruses, 26 of which encoded new amino acids. The highest percentage of changes was found in the E2 protein that potentially changed the antigenic properties of the viruses. This study gives new insight into BDV and how cytopathic viruses arise.
Technical Abstract: The complete genome sequences of a cytopathic/noncytopathic pair of border disease viruses (BDV) that were isolated from a sheep with mucosal disease-like lesions were determined. The viruses had 50 nucleotide differences and the cytopathic virus possessed an insert of 285 nucleotides that was derived from the Jiv cellular transcript.