Inhibition of Salmonella Typhimurium by Anaerobic Cecal Bacteria in Media Supplemented with Lactate and Succinate

Authors: Hinton Jr, Arthur; Ingram, Kimberly

Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/5/2013
Publication Date: 1/27/2014
Citation: Hinton Jr, A., Ingram, K.D. 2014. Inhibition of Salmonella Typhimurium by Anaerobic Cecal Bacteria in Media Supplemented with Lactate and Succinate. Southern Poultry Science Society. International Poultry Scientific Forum, Atlanta, Ga. January 27-29, 2014.

Interpretive Summary: None.

Technical Abstract: The ability of anaerobic cecal microflora of broilers to inhibit growth of Salmonella Typhimurium in media supplemented with lactate and succinate was examined. Cecal cultures were prepared by collecting ceca of processed broilers from a commercial processing facility, inoculating broth media with 10 µl of cecal contents, and incubating media anaerobically at 35oC for 48 h. Media supplemented with 0, 50, 100, or 150 mM of lactate and succinate were inoculated with 0.1 ml of the cecal culture, 104 cfu/ml of Salmonella, or the cecal culture and Salmonella Typhimurium. Media were incubated anaerobically at 35oC for 21 days, and aliquots of media were removed on Days 0, 7, 14, and 21 for analysis. Anaerobic cecal bacteria and Salmonella were enumerated, and cecal colonies were selected from agar and identified using the Biolog Bacterial Identification System. Results indicated that on Day 7, significantly (P < 0.05) fewer Salmonella were recovered from media supplemented with 100 or 150 mM of lactate and succinate and inoculated with cecal cultures and Salmonella than from media inoculated with Salmonella only. Additionally, on Day 14 significantly fewer Salmonella were recovered from media supplemented with 50 mM lactate and succinate and inoculated with cecal cultures and Salmonella than from media inoculated with Salmonella only. There was no significant difference in Salmonella recovered from media inoculated with cecal cultures and Salmonella or Salmonella only after 21 days of incubation in media not supplemented with lactate and succinate. Cecal isolates included Arcanobacterium, Abiotropia, and Gemella from media with 0 mM lactate and succinate; Lactococcus, Atopobium, and Peptostreptococcus from media with 50 mM lactate and succinate; Bacteroides, Lactococcus, and Arcanobacterium from media with 100 mM lactate/succinate; and Lactobacillus, Lactococcus and Streptococcus from media with 150 mM lactate and succinate. Findings indicate that cecal bacteria from processed broilers can utilize lactate and succinate to produce metabolites that inhibit growth of Salmonella. Understanding the anti-Salmonella activity of these bacteria may assist in identification of the bacterial isolates that may be included in effective probiotics. Key words: Broilers, competitive inhibition, cecal bacteria, lactate, succinate