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United States Department of Agriculture

Agricultural Research Service


Location: Animal Metabolism-Agricultural Chemicals Research

Title: Use of kidney inhibition swab test to evaluate antimicrobial residues in pork kidney from a market survey in Fargo, North Dakota

item Shelver, Weilin
item McGarvey, Amy
item Loeb, Erin -

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: March 5, 2014
Publication Date: N/A

Interpretive Summary: A commercially available on-site antibiotic test, the Kidney Inhibition Swab Test (KIS™ Test), has recently been adopted by the USDA Food Safety and Inspection Service (FSIS) to be used at slaughter plants. The KIS™ test was used in this study to determine whether antibiotics accumulated in pork kidneys used for human consumption. Our findings indicate that pork kidney sold in the local market for human consumption contained low/no antibiotics using the newly adapted USDA-FSIS antimicrobial screening test.

Technical Abstract: Introduction: The USDA-Food Safety and Inspection Service (FSIS) recently changed the on-site antimicrobial screening method to the Kidney Inhibition Swab (KIS™) test to be used at all livestock slaughter establishments. Although the newly adapted screening method can detect different classes of antibiotics, the test is most sensitive towards penicillin. Penicillin is one of the most commonly found tissue residue violations in annual USDA-FSIS surveys of pork. Previously, our study indicated that sows treated with label dose of penicillin G and slaughtered at the proper labeled-indicated withdrawal day tested positive when kidney was monitored with the KIS™ test. Purpose: The purpose of this study was to determine the extent of antimicrobial residues detected by the KISTM test in pork kidney obtained from local markets. Methods: Pork kidneys were purchased from two chain grocery stores and two ethnic specialty stores every 2-4 weeks to ensure that the kidneys obtained were from different lots. Although kidneys were obtained from local grocery stores, pork samples were obtained from suppliers in the entire mid-western region of North America. The KIS™ test were performed and color changes of the tests were recorded after prescribed incubation time at 64 ± 2 °C. A purple/blue color indicated antibiotics were present in the samples while yellow/green color indicated no/low antibiotics present in the samples. A “caution” indicates a sample contains antibiotics below the “positive” threshold. Results: One sample returned “caution” from our market survey. One “positive” sample was identified, but was not confirmed with the follow up tests. Significance: Our findings indicate that pork kidney in the market for human consumption contained low/no antibiotics using the newly adapted USDA-FSIS antimicrobial screening test.

Last Modified: 10/22/2014
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