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United States Department of Agriculture

Agricultural Research Service

Research Project: Microbial Ecology of Human Pathogens Relative to Poultry Processing

Location: Bacterial Epidemiology and Antimicrobial Resistance

Title: Antimicrobial Effect of An Essential Oil Blend on Surface-attached Salmonella on Polyvinyl Chloride

Authors
item Song, Sangyoon -
item Alali, Walid -
item Frank, Joseph -
item Berrang, Mark
item Hofacre, Charles -

Submitted to: International Association for Food Protection Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 5, 2014
Publication Date: N/A

Technical Abstract: The majority of drinking water lines for broilers are made of polyvinyl chloride (PVC) and surface attachment of Salmonella on the inner surface of water lines can be the initial stage of biofilm development. These biofilms can be the source of Salmonella infection in water lines and are known to resist many antimicrobial compounds. Essential oils (EO) have antimicrobial properties and can be mixed with water at low concentrations to deactivate Salmonella attached to PVC surfaces. The objective of this study was to determine the effect of an essential oil blend on Salmonella attached to PVC coupon surfaces compared to chlorine and untreated control. Thirty sterilized PVC Type 1 coupons (2 X 2 cm) were incubated in 2.2 × 108 CFU/mL of Salmonella strain cocktail (Enteritidis, Heidelberg, and Typhimurium) in TSB for 96 h at 37°C at 100 rpm to develop Salmonella attachment. Coupons (n=10/group) with attached Salmonella were treated with EO (0.05%), sodium hypochlorite (5ppm), or deionized water, and incubated at 25°C for 24 h at 100 rpm. After rinsing with PBS, coupon surfaces were swabbed using a sterile sponge, then stomached in 50mL of Dey-Engley neutralizing broth for 1 min at 260 rpm, and enumerated on XLT-4 plates and enriched for Salmonella (when direct plating was negative). There were no Salmonella colonies detected via direct plating and enrichment on PVC coupons treated with EO or sodium hypochlorite. The average log10 CFU/cm2 (± standard error) of the control was 1.4 ± 0.34, which was significantly higher than treated groups. The EO mixture and chlorine with water may be used to deactivate Salmonella attached to PVC surfaces.

Last Modified: 12/19/2014
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