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United States Department of Agriculture

Agricultural Research Service

Research Project: Epidemiology and Management of Pierce's Disease and Other Maladies of Grape

Location: Crop Diseases, Pests and Genetics

Title: A whole genome sequence of ‘Candidatus Liberibacter asiaticus’ from Guangdong, China, where HLB was first described

Authors
item Zheng, Zheng -
item Deng, Xiaoling -
item Chen, Jianchi

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 9, 2014
Publication Date: N/A

Technical Abstract: Citrus Huanglongbing (HLB, yellow shoot disease) has been endemic in Guangdong Province, China, for >100 years. “Candidatus Liberibacter asiaticus” (CLas) is a putative pathogen of HLB and currently unculturable. Here, a draft whole genome sequence of CLas strain A4 from Guangdong is presented. Strain A4 was originally collected from an HLB affected mandarin citrus (Citrus reticulata cultivar Shatangju) in Sihui City of Guangdong Province and transmitted to another Shatangju plant through grafting in 2005. In 2007, CLas was transmitted from citrus to periwinkle (Catharanthus roseus) via dodder (Cuscuta campestris). For sequencing, DNA was extracted from CLas-infected periwinkle tissue and sequenced using Illumina technology after removing methylated DNA and multiple displacement amplification. Sequence reads of CLas were extracted from the data by reference to currently available whole genome sequences of CLas obtained from infected psyllid vectors. The assembled A4 genome consisted of 1,208,625 bp, with G+C content of 36.4%, 1,107 predicted open reading frames, and 53 RNA genes. The A4 genome sequence has been deposited at DDBJ/EMBL/GenBank under the accession no. JFGQ00000000. In addition to originating from the historical HLB endemic region, the A4 whole genome sequence is also the first from a plant host.

Last Modified: 10/25/2014
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