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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Enhancement of Allium, Cucumis, and Daucus Germplasm

Location: Vegetable Crops Research Unit

Title: Genetic basis of mitochondrial sorting in cucumber

Authors
item Del-Valle Echevarria, Angel -
item Sanseverino, Walter -
item Tormo, Marc -
item Garcia-Mas, Jordi -
item Al-Faifi, Sulieman -
item Calderon, Claudia -
item Havey, Michael

Submitted to: Cucurbitacea
Publication Type: Abstract Only
Publication Acceptance Date: April 2, 2014
Publication Date: N/A

Technical Abstract: Regeneration of cucumber from cell cultures produces plants with distinct mosaic (MSC) phenotypes, misshapen cotyledons and leaves, reduced fertility, and low seed germination. The MSC phenotypes are paternally transmitted and associated with deletions or under-representations of specific regions of their mitochondrial (mt) DNAs. Quantitative reverse-transcriptase PCR established that genes located in these regions show reduced transcription relative to wild-type plants. A nuclear locus, Paternal Sorting of Mitochondria (Psm), conditions sorting to wild-type mitochondria in progenies when MSC plants are used as pollen parents. We mapped Psm to chromosome 3 of cucumber and identified a pentatricopeptide repeat (PPR) as a candidate gene controlling mitochondrial sorting in cucumber. This research demonstrates the usefulness of cucumber as a model plant to study nuclear-organellar interactions.

Last Modified: 10/26/2014
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