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United States Department of Agriculture

Agricultural Research Service

Research Project: Resources for the Genetic Improvement of Potato

Location: Vegetable Crops Research Unit

Title: Progress toward the development of recombinant inbred lines in potato

Authors
item Jansky, Shelley
item Douches, David -
item Endelman, Jeff -

Submitted to: American Journal of Potato Research
Publication Type: Proceedings
Publication Acceptance Date: March 27, 2014
Publication Date: N/A

Technical Abstract: Complexities due to tetraploid genetics limit breeding progress in potato. Diploids offer more simple genetics. Homozygous populations such as recombinant inbred lines are powerful resources for genetic mapping and the subsequent development of markers for marker-assisted selection. Most potato diploids are self-incompatible, but through the use of the dominant self-incompatibility inhibitor, Sli, we can create diploid inbred lines. We are developing sets of recombinant inbred lines (RILs) carrying genes from wild and cultivated species for improvement of tuber quality and disease resistance. Early generations (F2 and F3) are morphologically highly variable and exhibit severe inbreeding depression. Later generations (F4 and F5) are more uniform and vigorous. We are monitoring the phenotypic and genotypic effects of self-pollination to the F6 generation. Our goal is to create 100 F6 inbred lines of each of six populations. These will be shared with the research community for phenotyping and mapping.

Last Modified: 10/22/2014
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