Skip to main content
ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #313243
Title: Optimum conditions for the production of soy polyol oils and diacylglycerol from soybean oil by Acinetobacter haemolyticus A01-35 NRRL B-59985

Author
item HOU, CHING
item Ray, Karen

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/6/2015
Publication Date: 5/6/2015
Citation: Hou, C.T., Ray, K.J. 2015. Optimum conditions for the production of soy polyol oils and diacylglycerol from soybean oil by Acinetobacter haemolyticus A01-35 NRRL B-59985 [abstract].

Interpretive Summary:

Technical Abstract: Triacylglycerols (TAG) containing hydroxy fatty acids have many industrial uses such as the manufacture of aviation lubricant, plastic, paint, nylons, and cosmetics, because of the hydroxyl groups on the fatty acid (FA) constituents. Diacylglycerols (DAG) containing hydroxy FA can also be used in the above mentioned industries. Soy-polyols (oxygenated TAG) are important starting materials for the manufacture of polymers such as polyurethane. Recently we developed a new method to screen microorganisms for the direct production of polyol oils from soybean oil. One of the cultures, Acinetobacter haemolyticus A01-35 NRRL B-59985, produced DAGs containing hydroxyl FA and DAGs with normal FA from soybean oil (BAB 2: 1-6, 2013). Now we determined the optimum conditions for the production of these products as: pH 6.8; temperature 28 C; time 3 day. With galactose as carbon source it produced more polyol oils whereas with mannose it produced more DAG with normal FA. Yeast extract at 10g/L plus trypton at 5-7g/L serves as better nitrogen sources. The culture medium is best without NiCl2, CoCl2, or nicotinic acid. With optimum conditions, culture A01-35 produced 4.42 mg polyol oils and 23.31mg DAG with normal FA per 30ML culture medium.