Skip to main content
ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #316950
Title: Complete genome sequence of ‘Candidatus Liberibacter africanus’

Author
item Lin, Hong
item PIETERSEN, GERHARD - University Of Pretoria
item HAN, CLIFF - Los Alamos National Research Laboratory
item READ, D. ALAN - University Of Pretoria
item LOU, BINGHAI - Guangxi Citrus Research Institute
item GUPTA, GOUTAM - Los Alamos National Research Laboratory
item Civerolo, Edwin

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2015
Publication Date: 7/16/2015
Citation: Lin, H., Pietersen, G., Han, C., Read, D., Lou, B., Gupta, G., Civerolo, E.L. 2015. Complete genome sequence of ‘Candidatus Liberibacter africanus’. Genome Announcements. 3(4):300733-15. doi: 10.1128/genomeA.00733-15..

Interpretive Summary: Huanglongbing is a devastating disease of citrus caused by the bacterium, “Candidatus Liberibacter spp”. The complete genome sequence of ‘Candidatus Liberibacter africanus’ (Laf) strain ptsapsy was obtained by high through-put next generation sequencing technology. The Laf strain genome possesses a single circular chromosome of 1,192,232 nucleotides. A total 1,141 genes were predicted based on computational analysis. The complete genome sequence of Laf provides new information for identification of critical genes and facilitates development of effective strategies to control this economically important disease.

Technical Abstract: The complete genome sequence of ‘Candidatus Liberibacter africanus’ (Laf), strain ptsapsy, was obtained by an Illumina HiSeq 2000. The Laf genome comprises 1,192,232 nucleotides, 34.5% GC content, 1,141 predicted coding sequences, 44 tRNAs, 3 complete copies of ribosomal RNA genes (16S, 23S and 5S) and 368 hypothetical genes. In addition, two tandem aligned prophage segments were identified in the Laf genome. The average GC content in this prophage region is ~42.1%, deviating significantly from the core genome. This Laf genome sequence facilitates comparative genome analysis and advances in understanding of pathogenicity of citrus huanglongbing disease.