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United States Department of Agriculture

Agricultural Research Service

Title: Affinity Column Cleanup for the Analysis of Fumonisins and Their Hydrolysis Products in Corn

item Maragos, Chris
item Richard, John
item Bennett, Glenn

Submitted to: Pacific Basin Society Chemical International Congress Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 22, 1995
Publication Date: N/A

Technical Abstract: Fumonisins, toxic metabolites of certain Fusarium molds, can be found in corn and corn-based foods at levels sufficient to cause disease in livestock. The fumonisins have a variety of physical properties, such as anionic tricarballylic acid groups and a long carbon backbone, that make isolation of this group of compounds difficult. Widely used methods involve organic extraction followed by isolation with either C18 or strong anion exchange columns. We report the development of an affinity column procedure that is useful for isolation of both intact and hydrolyzed fumonisins from corn. The columns were prepared using two monoclonal antibodies with differing specificities, the first (P2A5-3-F3) bound intact fumonisins while the second (P2F11-3-H7) bound their hydrolysis products. Recoveries of fumonisin B1 from corn spiked with 0.25 to 2.5 ug/g averaged 82%, recoveries of hydrolyzed fumonisin B1 over the range of 0.25 to 2.5 ug/g averaged 85%. By using affinity columns capable of binding both chemical forms of fumonisins, the need for separate analytical procedures was eliminated.

Last Modified: 4/22/2015
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